Purdue UniversityPurdue Life Science Microscopy Facility
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 About Facility - Concept

The Life Science Microscopy Facility at Purdue University is a core facility under the umbrella of the campus-wide Purdue Electron Microscopy Consortium (PEMC). It is administered through Horticulture and Landscape Architecture (HLA) in the School of Agriculture. An advisory committee consisting of faculty from the Schools of Agriculture, Science, Pharmacy, Engineering, and Veterinary Medicine provides direction on facilities and procedures. Located in the Whistler Research Building, this 4000 sq. ft. laboratory complex supports research on microscopic and submicroscopicstructure of biological and physical systems and is available to the campus community.

Originally established in 1983 to consolidate ultrastructural technology in the Agricultural Complex, the LSMF has served research needs of a wide range of programs from basic molecular biology and cell biology to applied agricultural and engineering research. The main functions of the LSMF are to provide equipment, training (SEM and TEM courses), space, advice, and expertise so that researchers can come to the laboratories to conduct their research efficiently. There are both multi-user and service options available. The facility contains research equipment for use by students, faculty, postdoctorals, and service staff.

Technology in the LSMF provides capabilities for light microscopy, transmission electron microscopy, scanning electron microscopy, and computer-based image analysis. Equipment is available for cryo sample preparation (high pressure freezing and freeze substitution, ultramicrotomy, critical point drying, vacuum evaporation, sputter coating, digital printing, and histological and cytological specimen preparation.  Staff in the LSMF provides expertise in a wide range of specialized preparation techniques including immunocytochemistry and freeze substitution to assist researchers.