NSF-MCB The Genetics of Genetics: Genes controlling meiotic recombination in maize. (subawards to H. Dooner, Waksman Institute, W. Eggleston, Va. Commonwealth Univ., P. Schnable, Iowa St. Univ. and S. Stack, Colorado St. Univ.; on no-cost extension).
The major objective of this project has been to identify mutants that have altered rates and localizations of meiotic recombination events using marker alleles causing visible kernel phenotypes. We have succeeded in finding ~60 putative mutations, have thus far validated 10 of these (more are in progress) and are initiating mapping of these mutations. Once homozygous rec lines are established we will begin characterizing the placement and number of recombination nodules in these lines, whether their effects are global or restricted to specific regions of the genome and whether they influence the positioning of transposition events.