This is a listing of all procedure documents available at this time. If none of these documents provide adequate answers to your questions, click the contact us link to the left and submit your question via the provided form.

TEM

• FEI/Philips CM-10 TEM Alignment.doc

SEM

• JEOL-840 SEM Alignment.doc
• JEOL-840 Speciment Insertion.doc
• FEI NOVA nanoSEM

Light Microscopes

• Olympus Vanox instructions4.doc
• Nikon SMZ-U stereoscope instructions5.doc

Support Equipment

• 4- Reichert Ultracut E ultramicrotomes instructions.doc

TEM Sample Preparation-Fixation

• AGC Fixation-Bacteria/Yeast.doc This fixative is useful in fixing heavily walled organisms such as yeast and bacteria.
• Karnovsky's Fixative  Combination of paraformaldehyde and glutaraldehyde-often used for plant materials and other tissues that are difficult for fix to penetrate.
• Polysaccharide Fixation-RR+PATO
• Ruthinium Red / Lysine Fix  Used to preserve polysaccharides and other negatively charges glycocalyx materials
• Fixation Stock Solutions.doc
• Van Tuinen Fix

TEM Embedding

• Durcupan Resin.doc
• JB-4 Resin Instructions.doc 
• Lowicryl HM20 Method